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My CartReactivity：Human,Rat,Mouse
Application：WB,IHC
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：5609
Host：Rabbit
Qty：
Size：
| Name | Anti-MAP2K7 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:2000-5000. IHC 1:25-100. |
| Target | MAP2K7 |
| Background | Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K4/MKK4, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4/MKK4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The monophosphorylation of JNKs on the Thr residue is sufficient to increase JNK activity indicating that MAP2K7/MKK7 is important to trigger JNK activity, while the additional phosphorylation of the Tyr residue by MAP2K4/MKK4 ensures optimal JNK activation. Has a specific role in JNK signal transduction pathway activated by proinflammatory cytokines. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. Part of a non-canonical MAPK signaling pathway, composed of the upstream MAP3K12 kinase and downstream MAP kinases MAPK1/ERK2 and MAPK3/ERK1, that enhances the AP-1-mediated transcription of APP in response to APOE. |
| Swiss Prot | O14733 |
| Gene ID | 5609 |
| Subcellular Location | Cytoplasm Nucleus |
| Immunogen | A synthetic peptide of human MAP2K7 |
| Public Immunogen Range | 260-310/419aa |
| Calculated MW | 47/48/49/52kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Specification | 20ul 50ul 100ul |
| Reactivity | Human Rat Mouse |
| Application | WB IHC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with MAP2K7 antibody diluted at 1:4000;Lane: HL-60,A431,Mouse brain.
Immunohistochemistry of paraffin-embedded Human colorectal cancer with MAP2K7 antibody diluted at 1:50.
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