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Determination of Significance：
Creatinine (Cre), the chemical formula is C4H7N3O, is a product of muscle metabolism in the human body, which is mainly excreted by glomerular filtration. Serum creatinine comes from both exogenous and endogenous sources. Exogenous creatinine is the product of meat metabolism in the body; endogenous creatinine is the product of muscle metabolism in the body.
Measurement Principle：
The creatinine in the sample is hydrolyzed to creatine under the catalysis of creatinine enzyme. Under the catalysis of creatinase, creatine hydrolyzes to creatine and urea. Sarcosine is oxidized to hydrogen peroxide under the catalysis of sarcosine oxidase. Peroxidase catalyzed the oxidation of 4-aminoantipyrine coupling phenol by hydrogen peroxide to form colored compounds with characteristic absorption peak at 505nm.
Self Provided：
Spectrophotometer/microplate reader, low tempareture centrifuge, transferpettor, micro glass cuvette/96 well flat-bottom plate, mortar/homogenizer, ice and distilled water, ultrasonic crusher.
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