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Application：ELISA
Reactivity：Human
Sample Type：Serum，Plasma，Cell Culture Supernatant
Sensitivity：0.156 ng/ml
Storage：2-8℃，6 months
Gene ID：11026
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| Name | Human LILRA3 ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | LILRA3 |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Human |
| Application | ELISA |
| Reaction Type | Sandwich-ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 0.31-20 ng/ml |
| Sensitivity | 0.156 ng/ml |
| Specificity | Do not react with Human LILRA3/ILT6 analogue etc. |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Assay Time | 3h |
| Swiss Prot | Q8N6C8 |
| Gene ID | 11026 |
| Detection Principle | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗人LILRA3/ILT6单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的人LILRA3/ILT6会与酶标板上的包被抗体充分结合；洗板后加入HRP酶标记的抗人LILRA3/ILT6抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的人LILRA3/ILT6发生特异性结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的人LILRA3/ILT6，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的人LILRA3/ILT6浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中人LILRA3/ILT6的浓度。 |
| Background | ILT6，also known as LILRA3，belongs to the ILT family. In humans，the ILT gene family includes up to 11 members. The extracellular portion of all members includes at least two and usually four immunoglobulin domains. ILT-2 through 5 are all inhibitory members having variable numbers of cytoplasmic ITIM domains. ILT6 lacks a transmembrane domain. The function of ILT6 is currently unknown. however it is highly homologous to other LILR genes，and can bind human leukocyte antigen (HLA) class I. Therefore，if secreted，the ILT6 might impair interactions of membrane-bound LILRs (such as LILRB1，an inhibitory receptor expressed on effector and memory CD8 T cells) with their HLA ligands，thus modulating immune reactions and influencing susceptibility to disease. |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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