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My CartReactivity：Human,Rat,Mouse
Application：IHC,ELISA,IF
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：2182
Host：Rabbit
Qty：
Size：
| Name | Anti-ACSL4 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | IHC 1:50-200. IF 1:50-200. ELISA 1:3000-30000. |
| Target | ACSL4 |
| Background | Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoA for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially activates arachidonate and eicosapentaenoate as substrates. Preferentially activates 8,9-EET > 14,15-EET > 5,6-EET > 11,12-EET. Modulates glucose-stimulated insulin secretion by regulating the levels of unesterified EETs (By similarity). Modulates prostaglandin E2 secretion. |
| Swiss Prot | O60488 |
| Gene ID | 2182 |
| Subcellular Location | Cytoplasm Cell membrane |
| Immunogen | Recombinant protein of human ACSL4 |
| Public Immunogen Range | 1-350aa |
| Calculated MW | 79/74kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Y-Dilution Ratio | WB 1:2000-5000. IHC 1:50-200. ELISA 1:3000-30000. |
| Specification | 20ul 50ul 100ul |
| Reactivity | Human Rat Mouse |
| Application | IHC ELISA IF |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Immunohistochemistry of paraffin-embedded Human colorectal cancer with ACSL4 antibody diluted at 1:100.
Immunohistochemistry of paraffin-embedded Human endometrial cancer with ACSL4 antibody diluted at 1:100.
Immunofluorescence analysis of NIH3T3 cells using ACSL4 antibody diluted at 1:100.
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CA9 knockdown enhanced ionizing radiation-induced ferroptosis and radiosensitivity of hypoxic glioma cells
Click to check >>Author：Wenpeng Huang, Yuping He, Shuangyu Yang, Xuefei Xue, Hualong Qin, Ting Sun, Wei Yang
IF：2.6000
Publish_to：INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
PMID：37463506
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