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My CartReactivity：Human,Rat,Mouse
Application：WB,IHC,IF
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：8322
Host：Rabbit
Qty：
Size：
| Name | Anti-FZD4 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:1000-3000. IHC 1:50-200. IF 1:50-200. |
| Target | FZD4 |
| Background | This gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. This protein may play a role as a positive regulator of the Wingless type MMTV integration site signaling pathway. A transcript variant retaining intronic sequence and encoding a shorter isoform has been described, however, its expression is not supported by other experimental evidence. |
| Swiss Prot | Q9ULV1 |
| Gene ID | 8322 |
| Subcellular Location | Cell membrane Cytoplasm Nucleus |
| Immunogen | A synthetic peptide of human FZD4 |
| Public Immunogen Range | 110-160/537aa |
| Calculated MW | 60kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Specification | 20ul 50ul 100ul |
| Reactivity | Human Rat Mouse |
| Application | WB IHC IF |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with FZD4 antibody diluted at 1:2000;Lane: B16,HepG2,Mouse eye,Mouse trachea.
Immunohistochemistry of paraffin-embedded Human stomach cancer with FZD4 antibody diluted at 1:100.
Immunofluorescence analysis of Hela cells using FZD4 antibody diluted at 1:100.
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