{{cart_num}}
My CartReactivity：Human,Rat,Mouse
Application：WB,IHC,IF
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：55662
Host：Rabbit
Qty：
Size：
| Name | Anti-HIF1AN Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:1000-3000. IHC 1:50-200. IF 1:50-200. |
| Target | HIF1AN |
| Background | Hydroxylates HIF-1 alpha at 'Asn-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation. |
| Swiss Prot | Q9NWT6 |
| Gene ID | 55662 |
| Subcellular Location | Nucleus Cytoplasm |
| Immunogen | Recombinant protein of human HIF1AN |
| Public Immunogen Range | 1-350aa |
| Calculated MW | 40kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Specification | 20ul 50ul 100ul |
| Reactivity | Human Rat Mouse |
| Application | WB IHC IF |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with HIF1AN antibody diluted at 1:2000;Lane: 293T,HEK293,Jurkat,Mouse heart.
Immunohistochemistry of paraffin-embedded Human breast cancer with HIF1AN antibody diluted at 1:100.
Immunofluorescence analysis of Hela cells using HIF1AN antibody diluted at 1:100.
Sorry, there is no more information.
Sorry, there is no more information.
Manual Download
