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My CartReactivity：Human,Mouse
Application：WB,IF,IHC
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：1975
Host：Rabbit
Qty：
Size：
| Name | Anti-EIF4B Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:1000-3000. IHC 1:100-300. IF 1:50-200. |
| Target | EIF4B |
| Background | Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F. |
| Swiss Prot | P23588 |
| Gene ID | 1975 |
| Subcellular Location | Cytoplasm |
| Immunogen | A synthetic peptide of human EIF4B |
| Public Immunogen Range | 535-585/611aa |
| Calculated MW | 69/65kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Specification | 20ul 50ul 100ul |
| Reactivity | Human Mouse |
| Application | WB IF IHC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with EIF4B antibody diluted at 1:2000;Lane: Raw264.7,NIH3T3,MCF7,HeLa,293T,A549,K-562.
Immunohistochemistry of paraffin-embedded Human endometrial cancer with EIF4B antibody diluted at 1:200.
Immunofluorescence analysis of HepG2 cells using EIF4B antibody diluted at 1:100.
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