English
中文
Bis-Tris is a buffer with a pH buffer range of 5.8-7.2; An effective buffer for the determination of creatine kinase; A protective agent for hemoglobin freeze-drying.
Chinese name: Di (2-hydroxyethyl) imidotrimethylol methane
English name: Bis[2-Hydroxyethyl] imino Tris-(Hydroxymethyl)-methane
CAS:6976-37-0
Molecular formula: C8H19NO5
Molecular weight: 209.2
Grade: Biochemical grade
Quality standard:
Appearance: White crystal
Content: ≥ 99.0%
Melting point: 100.0-104.0℃
Water soluble: Transparent
PH(1M aqueous solution,20℃) : 9.0-11.0
Application: In biological experiments, it is an important PH stabilization reagent, usually select the appropriate weak acid and its conjugate base mix to get the appropriate PH value. Most biological reactions occur under neutral conditions, the general PH is selected between 6-8, and the effective buffer range of the buffer is also required between 6-8. In addition, the acid-base form of the buffer is required not to chelate with some metal ions. It is also used to detect creatine creatinase, which effectively protects hemoglobin in freeze-drying. Cathode electrolyte for IEF/2-D gel electrophoresis.
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