Gene Name |
PSMC1 |
English Name |
proteasome (prosome, macropain) 26S subunit, ATPase, 1 |
Chinese Name |
ATP酶蛋白酶体26S亚基1 |
Uniprot |
P62191 |
GeneID |
5700 |
Predicted Cross Reactivity |
Human Mouse Rat (Chicken) |
氨基酸大小 |
39kDa |
表达氨基酸 |
93-440aa |
Background |
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator.The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits.The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits.Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway.An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides.This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity.This subunit and a 20S core alpha subunit interact specifically with the hepatitis B virus X protein, a protein critical to viral replication.This subunit also interacts with the adenovirus E1A protein and this interaction alters the activity of the proteasome.Finally, this subunit interacts with ataxin-7, suggesting a role for the proteasome in the development of spinocerebellar ataxia type 7, a progressive neurodegenerative disorder. |
Application |
Immunology research |
Source |
E.coli |
Purification |
NI-NTA affinity purification |
Formulation |
Lyophilized from a 0.2 μm filtered solution of Urea, Tris-HCl, PH8.0. |
Delivery Condition |
The product is shipped at -20°C temperature. Upon receipt, store it immediately at the temperature listed below. |
Storage |
Reconstituted protein solution can be stored at 4-7°C for 1-2 months, stable at < -20°C for 2-3 years. |
Reconstitution |
Reconstituted protein solution can be diluted with distilled PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Purity |
Purity≥85% as determined by reducing SDS-PAGE. |
Bioactive |
Not tested. |
Tag information |
With a 6His tag at the N-terminus. |
Unit |
Piece |
Specification |
10ug 50ug 100ug 1mg |