Glycosylated Serum Protein Content Assay Kit( NBT-Colorimetric Method)
Cat.No:BC4945 Solarbio
Detection Method:Spectrophotometer/Microplate Reader
Storage:Store at -20℃,6 months
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Glycosylated Serum Protein Content Assay Kit( NBT-Colorimetric Method)Detection Method:Spectrophotometer/Microplate Reader
Storage:Store at -20℃,6 months
Qty:
Size:
Name | Glycosylated Serum Protein Content Assay Kit( NBT-Colorimetric Method) |
Detection Method | Spectrophotometer/Microplate Reader |
Storage | Store at -20℃,6 months |
Unit | Kit |
Specification | 100T/96S |
Determination of Significance:
Serum glucose reacts non-enzymatically with the N-terminal amino group of albumin and other serum protein molecules to form a polymer ketamine structure. Under alkaline conditions, it reacts with nitrotetrachloroazole blue to produce formazan, a purple-red compound. Comparing the color at 530 nm wavelength, measuring its OD value. And comparing with DMF standard, the content can be obtained.
Measurement Principle:
Serum glucose reacts non-enzymatically with the N-terminal amino group of albumin and other serum protein molecules to form a polymer ketamine structure. Under alkaline conditions, it reacts with nitrotetrachloroazole blue to produce formazan, a purple-red compound. Comparing the color at 530 nm wavelength, measuring its OD value. And comparing with DMF standard, the content can be obtained.
Self Provided:
Spectrophotometer/microplate reader, desk centrifuge, transferpettor, mico glass cuvette/96 well flat -bottom plate, mortar/homogeniser, ice and distilled water.
Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark:These protocols are for reference only. Solarbio does not independently validate these methods.
Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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