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Determination of Significance：
Trehalose is a functional oligosaccharide. It has the characteristics of non-reduction, moisture retention, heat the acid stability, anti-freezing and so on. It is one of the important anti stress substances produced by cells in adverse environmental conditions. It has nonspecific protective effect on biological macromolecules and tissues.
Measurement Principle：
Trehalose synthase (TS) can catalyze maltose to trehalose. It is one of the key pathways of trehalose biosynthesis. In this kit, the remaining maltose is decomposed into glucose by glucoamylase. Use the glucose oxidase method to determine the glucose content. The activity of trehalose synthetase can be shown by the reduced amount of maltose.
Self Provided：
Spectrophotometer, desk centrifuge, transferpettor, 1 mL glass cuvette, mortar/homogeniser, ice and distilled water.
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