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My Cart> Biochemical Assay Kits > Nitrogen Metabolism Series > Other Nitrogen Metabolism Series > Nitrate Reductase (NR)Activity Assay Kit（Griess-Colorimetric Method）Detection Method：Spectrophotometer/Microplate Reader
Storage：Store at 2-8℃，6 months
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| Name | Nitrate Reductase (NR)Activity Assay Kit（Griess-Colorimetric Method） |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at 2-8℃，6 months |
| Unit | Kit |
| Specification | 100T/48S |
Determination of Significance：
NR (EC 1.7.1.3) is a key enzyme in the transformation of plant nitrate nitrogen into ammonia nitrogen as well as an induction enzyme, which widely exists in plants and has an impact on crop yield and quality.
Measurement Principle：
NR catalyzed nitrate reduction to nitrite, with NO3ˉ +NADH+H＋→NO2ˉ+NAD＋+H2O. In the acidic conditions, the NO2ˉ produced can participate in the diazotization reaction to produce a purple-red compound, which has an absorption peak at 540 nm, and the change in absorbance value at 540 nm indicates the enzyme activity.
Self Provided：
Spectrophotometer/microplate reader, water bath/incubator, benchtop centrifuge, micro glass cuvettes/96 well plates, mortar/ homogenizer, ice and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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