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Fast endonuclides are a series of restriction endonuclides that have been genetically engineered to precisely cut DNA in 5 to 15 minutes, and are suitable for rapid enzyme cutting of plasmid DNA, PCR products or genomic DNA. Fast endonuclease has the following characteristics: it can be completed in 5~15 minutes; Sharing one digestion Buffer greatly simplifies the digestion reaction system. Good redundancy of enzyme activity, easy to cope with substrate overload or difficult template enzyme cutting. In addition, dephosphorylation and ligation reagents are 100% active in the digestion Buffer, supporting a tubeization reaction and improving the enzyme-modified-ligation experience.
REF: MD045
5 '... A /A G C T T... 3 '
3 '... T T C G A/ A... 5 '
Fast enzyme that can complete the reaction in 5-15 minutes
Reaction conditions: 10× FastCut Buffer&10× FastCut Color Buffer, 37℃
Thermal inactivation: 80℃for 20 minutes
Methylation sensitivity: Influenced by EcoBI methylation, clipping may be affected when sequences overlap
Asterisk activity: 1 hour incubation does not show asterisk activity, 16 hours may show asterisk activity
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
