Fast endonuclides are a series of genetically engineered restriction endonuclides that can precisely cut DNA within 5 to 15 minutes, and are suitable for fast enzyme cutting of plasmid DNA, PCR products or genomic DNA. Fast endonuclease has the following characteristics: It can be completed in 5 to 15 minutes; Sharing one digestion Buffer greatly simplifies the digestion reaction system. Good redundancy of enzyme activity, easy to cope with substrate excess or difficult template enzyme cutting. In addition, dephosphorylation and ligation reagents are 100% active in the digestion Buffer, supporting a tubeization reaction and improving the enzyme-modified-ligation experience.
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