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Biochemical reagents for scientific research.
Source: Cattle source
Instructions for use:
The optimal coating concentration depends on the cell type and the purpose of application or study.
1. Dissolve collagen with 0.1M acetic acid solution and configure a 0.1% (w/v) collagen storage solution. Room temperature stirring
1-3h until the collagen is completely dissolved.
2. Take a sterile glass bottle, line the bottom of the bottle with a layer of chloroform, transfer the collagen solution to the top layer of chloroform, chloroform volume
It is 10% of the collagen solution and cannot be shaken or stirred. Let stand overnight at 2-8℃. Transfer the upper layer collagen solution
To a sterile container (sterile operation). If the filter membrane is used to filter the bacteria, the protein will be lost.
3. Calculate the amount of coated liquid required according to the coated area; The 0.1% collagen storage solution was diluted 10-fold to produce a working concentration of 0.01%.
4. Add collagen working solution to the cell culture dish at a concentration of 6-10ug/cm2 and cover the surface completely.
5. Leave at room temperature or 37℃ for several hours, or 2-8℃ overnight.
6. Blot away excess liquid and allow petri dishes to dry overnight. A good petri dish surface can be sterilized overnight by UV irradiation.
7. The surface of the cell culture dish can be washed with a sterile balanced salt solution before reinoculating the cells.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
请问胶原蛋白Ⅰ型(可溶）C8061是否可以用作凝胶2022-10-12 09:53:06
Administrator您好，这个是做包被用的，制备三维胶凝胶可以用C8062、C8063、C8065
使用说明中的步骤3，十倍稀释配置工作液，请问用什么进行稀释2022-09-29 22:35:46
Administrator初始胶原蛋白是用醋酸溶液溶解的，稀释还是用这个，但要注意稀释液除菌使用。
请问胶原蛋白Ⅰ型(可溶）C8061来源是什么，是否是Ⅰ型鼠尾胶原2022-08-23 10:58:11
Administrator来自牛跟腱，不是Ⅰ型鼠尾胶原
胶原蛋白Ⅰ型(可溶）C8061是否可以跑SDS-PAGE ,分子量多大？是否可以用SDS-PAGE凝胶检测2022-08-05 11:36:17
Administrator您好，这一款胶原蛋白是做包被用的，没有跑过SDS-PAGE或者鉴定过分子量大小了，是天然来源的不是表达的重组蛋白