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My CartReactivity：Mouse,Human
Application：WB,IF
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：7291
Host：Rabbit
Qty：
Size：
| Name | Anti-TWIST1 Rabbit Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:100-500. IF 1:50-200. |
| Target | TWIST1 |
| Background | Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. The protein encoded by this gene is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Dermo1. The strongest expression of this mRNA is in placental tissue; in adults, mesodermally derived tissues express this mRNA preferentially. Mutations in this gene have been found in patients with Saethre-Chotzen syndrome. |
| Swiss Prot | Q15672 |
| Gene ID | 7291 |
| Subcellular Location | Nucleus |
| Immunogen | A synthetic peptide of human Twist |
| Public Immunogen Range | 51-150aa |
| Calculated MW | 20kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.03% Proclin300，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Mouse Human |
| Application | IF WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with TWIST1 antibody diluted at 1:1000;Lane: 293T cells.
Immunofluorescence analysis of NIH3T3 cells using TWIST1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining
Immunofluorescence analysis of HeLa cells using TWIST1 antibody diluted at 1:100.
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