CollagenaseⅠ
Cat.No：C8140 Solarbio
CAS：9001-12-01
Appearance：Brown crystalline powder
Storage：Store at -20℃，2 years
Purity：Degree of carboxylation 87-90%.
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Secondary Antibodies
SAlexa Fluor-Conjugated
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Other-Conjugated
Colloidal Gold Reagent
Alkaline Phosphatase(AP)-Conjugated
Colloidal Gold-Conjugated
FITC-Conjugated
Biotin-Conjugated
Purified Secondary Antibodies
Allophycocyanin(APC)-Conjugated
CY Fluorescent-Conjugated
Phycoglobin(PE)-Conjugated
RBTIC-Conjugated
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HIF
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Cytoskeleton
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Ubiquitin
JAK/STAT
GPCR & G Protein
Neuronal Signal Pathway
MAPK
NF-κB
Membrane Transporter & Ion Channel
TGF-β/Smad
Hippo
Antibody-drug Conjugate/ADC Related
Others
Inhibitors & Antagonists & Agonists
PI3K/Akt/mTOR
Endocrinology & Hormones
Protein Tyrosine Kinase/PTK
Angiogenesis
HIF
Immunology & Inflammation
Stem Cells
Apoptosis
Metabolic Enzyme & Protease
Autophagy
ER Stress
Cell Cycle
Cytoskeleton
DNA Damage & DNA Repai
Epigenetics
Ubiquitin
JAK/STAT
GPCR & G Protein
Neuronal Signal Pathway
MAPK
NF-κB
Membrane Transporter & Ion Channel
TGF-β/Smad
Hippo
Antibody-drug Conjugate/ADC Related
Others
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Pipette & Dispenser & Pipette Dispensing Instrument
Oscillating Mixing & Stirring & Dispersing Instrument
Temperature Control & Sampling & Grinding Crushing Instrument
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PCR Instrument & Light Glue Instrument
Spectrophotometer
Centrifuge
Shaker
Vacuum Pump
Other Small Instruments
Instruments
Pipette & Dispenser & Pipette Dispensing Instrument
Oscillating Mixing & Stirring & Dispersing Instrument
Temperature Control & Sampling & Grinding Crushing Instrument
Sterilization & Filtration Instruments
PCR Instrument & Light Glue Instrument
Spectrophotometer
Centrifuge
Shaker
Vacuum Pump
Other Small Instruments
Product Information
| Name | CollagenaseⅠ |
| CAS | 9001-12-01 |
| Storage | Store at -20℃，2 years |
| Appearance | Brown crystalline powder |
| Purity | Degree of carboxylation 87-90%. |
| Source | Collagenase from Clostridium histolyticum |
| Enzyme Activity/Titer | ≥125units/mg solid |
| Unit | Piece |
| Specification | 100mg 1g |
Product introduction
Collagenase I is a black brown powder in appearance. With a molecular weight of 68,000 to 125,000, collagens are able to specifically bind peptide bonds between neutral amino acids (X) and glycine in the -R-Pro-8-X-Gly-pro-R - sequence. The crude product is a mixed enzyme secreted by Clostridium histolytica. They are mainly collagenase, neutral protease and clostridium protein enzyme. Clostridium protease is the least active enzyme that is oxidized, and the separation of tissues must rely on the joint action of collagenase and neutral protease. Activator: Ca2+, the best concentration is 5 mM. Substrate: Different types of collagen are its natural substrates, and some synthetic peptides can also be used as substrates, such as: N-cbz-gly-pro-gly-pro-ala (Km = 0.71 mM), N-CBz-gly-pro-leu-Gly-Pro, N-(3-(2-furyl)acryloyl)- leu-gly-pro-ala(FALGPA), 4-Phenylazo benzyloxycarbonyl-pro-leu-gly-pro-D-arg, n-2,4-Dinitrophenyl- pro-gln-gly-ile-ala-gly-gln-D-arg. In addition, N-Succinyl-gly-pro-leu-glypro7-amido-4-methylcoumarin is a substrate of collagenase-like peptidase. N-(2, 4-dinitrophenyl) -Proleu-gly-Leu-TRp-Ala-D-ARG amino acid compounds are substrates for collagenase in vertebrates.
Scope of application
It is suitable for the isolation of epithelial, lung and adrenal tissue cells.
Usage method
Collagenase I derived from Clostridium tissotica can be formulated as 1-2 mg/ml with TESCA buffer (50 mM TES, 0.36 mM calcium chloride, pH 7.4, 37℃) (Cat:G0150). It can also be prepared with PBS containing calcium and magnesium, hanks containing calcium and magnesium, or serum free medium. After distribution, to avoid repeated freeze-thaw.
Unit definition
1 collagen digestion unit (CDU):at 37℃,pH 7.4 ,and in the presence of calcium ions, polypeptide equivalent to ninhydrin chromogenic 1 micromolar leucine released from collagen every 5 hours is an active unit.
1FALGPA unit: At 25℃, pH 7.5 and in the presence of calcium ions,one FALGPA hydrolysis unit can hydrolyze 1.0μmol of furanyl acrylyl-Leu-gly-pro-Ala per minute.
Optimum pH
6.3-8.8
Inhibitors
EGTA, Reduced Glutathione, β-Mercaptoethanol, Sodium Thioglycolate, 8-Hydroxyquinoline, 2,2' -Bipyridine etc.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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