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Determination of Significance：
α-Mannosidase is widely distributed and has many kinds. It is found in eukaryotic cytoplasm, endoplasmic reticulum, Golgi apparatus, and lysosome. Different types and functions of α-Man participate in the modification process of N-glycans.
Measurement Principle：
α-Mannosidase reacts with a specific substrate, and the product has a characteristic absorption peak at 405nm. The α-man activity can be calculated according to the rate of change in absorbance.
Self Provided：
Ultraviolet spectrophotometer/microplate reader, desk centrifuge, pipette, constant temperature incubator/water bath, micro quartz cuvette/96 well UV well plate, mortar/homogenizer, ice and distilled water.
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