Determination of Significance：

Serum amylase (AMY) belongs to α-amylase, which hydrolyzes α-1,4 glycosidic bonds inside polysaccharide molecules in a random manner to generate a mixture of oligosaccharides, maltose and glucose. AMY is mainly secreted by the salivary glands and pancreas, and a small amount of it is secreted by organs such as the proximal duodenum, lungs, uterus, and breast during lactation.

Measurement Principle：

Amylase catalyzes the hydrolysis of α-1,4 glycosidic bonds in starch molecules to produce glucose, maltose, dextrin, etc. Iodine can be combined with starch that is not hydrolyzed by amylase to form a complex with a characteristic absorption peak at 570 nm. The depth can calculate the unit of amylase activity. α-amylase is acid-resistant and β-amylase is heat-resistant. According to the above characteristics, the activity of another amylase can be measured by passivating one of them.

Self Provided：

Spectrophotometer/microplate reader, water bath/ incubator, desktop centrifuge, transferpettor, mortar/ homogenizer, micro glass cuvette/96 well UV plate, mortar, distilled water.

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