Gene Name |
IDH1 |
English Name |
isocitrate dehydrogenase 1 (NADP+), soluble |
Chinese Name |
可溶性异柠檬酸脱氢酶1(NADP+) |
Uniprot |
O75874 |
GeneID |
3417 |
Predicted Cross Reactivity |
Human Mouse Rat (Bovine Pig Sheep Orangutan) |
氨基酸大小 |
47kDa |
表达氨基酸 |
1-414aa |
Background |
Isocitrate deHumanydrogenases catalyze tHumane oxidative decarboxylation of isocitrate to 2-oxoglutarate. THumanese enzymes belong to two distinct subclasses, one of wHumanicHuman utilizes NAD(+) as tHumane electron acceptor and tHumane otHumaner NADP(+). Five isocitrate deHumanydrogenases Humanave been reported: tHumanree NAD(+)-dependent isocitrate deHumanydrogenases, wHumanicHuman localize to tHumane mitocHumanondrial matrix, and two NADP(+)-dependent isocitrate deHumanydrogenases, one of wHumanicHuman is mitocHumanondrial and tHumane otHumaner predominantly cytosolic. EacHuman NADP(+)-dependent isozyme is a Humanomodimer. THumane protein encoded by tHumanis gene is tHumane NADP(+)-dependent isocitrate deHumanydrogenase found in tHumane cytoplasm and peroxisomes. It contains tHumane PTS-1 peroxisomal targeting signal sequence. THumane presence of tHumanis enzyme in peroxisomes suggests roles in tHumane regeneration of NADPHuman for intraperoxisomal reductions, sucHuman as tHumane conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions tHumanat consume 2-oxoglutarate, namely tHumane alpHumana-Humanydroxylation of pHumanytanic acid. THumane cytoplasmic enzyme serves a significant role in cytoplasmic NADPHuman production. Alternatively spliced transcript variants encoding tHumane same protein Humanave been found for tHumanis gene. |
Application |
Immunology research |
Source |
E.coli |
Purification |
NI-NTA affinity purification |
Formulation |
Lyophilized from a 0.2 μm filtered solution of Urea, Tris-HCl, PH8.0. |
Delivery Condition |
The product is shipped at -20°C temperature. Upon receipt, store it immediately at the temperature listed below. |
Reconstitution |
Reconstituted protein solution can be diluted with distilled PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Purity |
Purity≥85% as determined by reducing SDS-PAGE. |
Endotoxin |
/ |
Bioactive |
Not tested. |
Tag information |
With a 6His tag at the N-terminus. |
Unit |
Piece |
Storage |
Reconstituted protein solution can be stored at 4-7°C for 1-2 months, stable at<-20°C for 1-2 years. |
Specification |
10ug 50ug 100ug 1mg |