Ultrafiltration centrifugal tube

Material: polypropylene cap, polypropylene tube, regenerated cellulose membrane, styrene-butadiene housing

Quality Level: 400

Sterility: non-sterile

Product line: Amicon®

Manufacturer/tradename:Amicon® Ultra

Parameter: 0.5 mL sample volume

Technique(s):protein extraction: suitable, protein purification: suitable

L: 4.99 cm (1.96 in.)

Diam.: 10.8 mm

Filtration area: 1 cm2

Volume: 0.5 mL

Pore size:3kDa NMWCO

Shipped in: ambient

General description:

Amicon® Ultra-0.5 centrifugal filter devices provide fast ultrafiltration, with the capability for high concentration factors and easy concentrate recovery from dilute and complex sample matrices. For example, Cytochrome c (12.4 kDa) at 0.25 mg/ml initial concentration is typically retained at >95%.

The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 30-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. Efficient recovery of the concentrated sample (retained species) is achieved by a convenient reverse spin step after collecting the filtrate.

Amicon® Ultra-0.5 devices are supplied non-sterile and are for single use only. With their vertical membrane design and reverse spin, Amicon® Ultra 0.5 mL filters deliver great performance and lower spin times.

Application:

1 Ultrafiltration with 3,000 Dalton molecular weight cutoff Ultracel® membrane

2 Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms

3 Purification of macromolecular components found in tissue culture extracts or cell lysates, primer removal, linkers, or macromolecular labels from a reaction mix, and protein removal prior to HPLC

4 Desalting, buffer exchange, and protein dialysis

Features and Benefits:

1 Ultracel® regenerated cellulose membrane with 3kDa NMWCO delivers retentate recovery of greater than 90%

2 Concentrates 500 μL down to 15 μL

3 Concentration factor 25x to 30x

4 High sample recovery (greater than 90% of dilute starting solution)

5 Fast processing time: 10 to 30 minutes

6 Reverse spin capability provides consistent recoveries

Physical form:

Color Code: Clear

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.