Material: polypropylene cap, polypropylene tube, regenerated cellulose membrane, styrene-butadiene housing

Quality Level: 400

Sterility: non-sterile

Product line: Amicon®

Manufacturer/tradename:Amicon® Ultra

Parameter: 0.5 mL sample volume

Technique(s):protein extraction: suitable, protein purification: suitable

L: 4.99 cm (1.96 in.)

Diam.: 10.8 mm

Filtration area: 1 cm2

Volume: 0.5 mL

Pore size:3kDa NMWCO

Shipped in: ambient

General description:

Amicon® Ultra-0.5 centrifugal filter devices provide fast ultrafiltration, with the capability for high concentration factors and easy concentrate recovery from dilute and complex sample matrices. For example, Cytochrome c (12.4 kDa) at 0.25 mg/ml initial concentration is typically retained at >95%.

The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 30-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. Efficient recovery of the concentrated sample (retained species) is achieved by a convenient reverse spin step after collecting the filtrate.

Amicon® Ultra-0.5 devices are supplied non-sterile and are for single use only. With their vertical membrane design and reverse spin, Amicon® Ultra 0.5 mL filters deliver great performance and lower spin times.

Application:

1 Ultrafiltration with 3,000 Dalton molecular weight cutoff Ultracel® membrane

2 Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms

3 Purification of macromolecular components found in tissue culture extracts or cell lysates, primer removal, linkers, or macromolecular labels from a reaction mix, and protein removal prior to HPLC

4 Desalting, buffer exchange, and protein dialysis

Features and Benefits:

1 Ultracel® regenerated cellulose membrane with 3kDa NMWCO delivers retentate recovery of greater than 90%

2 Concentrates 500 μL down to 15 μL

3 Concentration factor 25x to 30x

4 High sample recovery (greater than 90% of dilute starting solution)

5 Fast processing time: 10 to 30 minutes

6 Reverse spin capability provides consistent recoveries

Physical form:

Color Code: Clear