English
中文
Material: polypropylene cap, polypropylene tube, regenerated cellulose membrane, styrene-butadiene housing
Quality Level: 400
Sterility: non-sterile
Product line: Amicon®
Manufacturer/tradename:Amicon® Ultra
Parameter: 0.5 mL sample volume
Technique(s):protein extraction: suitable, protein purification: suitable
L: 4.99 cm (1.96 in.)
Diam.: 10.8 mm
Filtration area: 1 cm2
Volume: 0.5 mL
Pore size:3kDa NMWCO
Shipped in: ambient
General description:
Amicon® Ultra-0.5 centrifugal filter devices provide fast ultrafiltration, with the capability for high concentration factors and easy concentrate recovery from dilute and complex sample matrices. For example, Cytochrome c (12.4 kDa) at 0.25 mg/ml initial concentration is typically retained at >95%.
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 30-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. Efficient recovery of the concentrated sample (retained species) is achieved by a convenient reverse spin step after collecting the filtrate.
Amicon® Ultra-0.5 devices are supplied non-sterile and are for single use only. With their vertical membrane design and reverse spin, Amicon® Ultra 0.5 mL filters deliver great performance and lower spin times.
Application:
1 Ultrafiltration with 3,000 Dalton molecular weight cutoff Ultracel® membrane
2 Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
3 Purification of macromolecular components found in tissue culture extracts or cell lysates, primer removal, linkers, or macromolecular labels from a reaction mix, and protein removal prior to HPLC
4 Desalting, buffer exchange, and protein dialysis
Features and Benefits:
1 Ultracel® regenerated cellulose membrane with 3kDa NMWCO delivers retentate recovery of greater than 90%
2 Concentrates 500 μL down to 15 μL
3 Concentration factor 25x to 30x
4 High sample recovery (greater than 90% of dilute starting solution)
5 Fast processing time: 10 to 30 minutes
6 Reverse spin capability provides consistent recoveries
Physical form:
Color Code: Clear
