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Product introduction
Collagenase Ⅳ contains at least 7 protease components with molecular weights ranging from 68 to 130KD.
Scope of application
Can digest a variety of tissues.
Usage method
The concentration of collagenase is generally 0.1-5 mg/ml, and the digestion process can be stirred to promote the digestion of the enzyme. Collagenase can cause a small amount of cell death in the process of digestion, and the general digestion time is 15min to several hours. If the concentration changes then the digestion time may be longer. The preferred Buffer during digestion is the Krebs Ringer Buffer containing calcium ions and BSA. In the process of digestion, if a large number of cells die, it is necessary to reduce the amount of enzymes, and at the same time, 0.5%BSA or 5-10% serum can be added to stabilize the state of cells and digestive effect. Sterilization of collagenase begins with centrifugation or removal of insoluble matter with a 0.8μm filter. The bacteria are then filtered with a 0.2μm filter. The combination of benzamidine hydrochloride and TLCK can bind to most of the proteases in this enzyme component and inhibit its activity, but they do not inhibit collagenase activity. Among them, the concentration of benzamidine hydrochloride is generally 1mM, and the concentration of TLCK is generally 0.100-0.135mM. It is worth noting that both cannot completely guarantee that all protease activity is inhibited.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
