Material: polyethylene cap,polyethylene liner,polypropylene tube,regenerated cellulose membrane,regenerated cellulose support,styrene-butadiene housing

Quality Level: 400

Sterility: non-sterile

Product line: Amicon®

Manufacturer/tradename: Amicon® Ultra

Parameter: 4mL sample volume

Technique(s): protein extraction: suitable, protein purification: suitable

L: 124mm (4.88in.)

Diam.: 17.3 mm

Filtration area: 3cm2

Volume: 4mL

Pore size:30kDa NMWCO

Shipped in: ambient

General description:

Amicon® Ultra 4 mL centrifugal devices with Ultracel® 30,000 Dalton molecular weight cutoff filters provide fast ultrafiltration, and reliable retention of macromolecules. For example, Ovalbumin (45kDa) at 1 mg/ml initial concentration is retained at >90% .

The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.

The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. With their vertical membrane design and deadstop, Amicon® Ultra 15mL filters deliver the highest recovery with short spin times.

Application:

1 Ultrafiltration with 30,000 Dalton molecular weight cutoff Ultracel® membrane.

2 Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.

3 Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLC.

4 Concentration of dilute or pre-purified proteins from column eluents.

5 Desalting, buffer exchange and protein dialysis.

6 Size exclusion and protein fractionation.

Features and Benefits:

1 Ultracel® regenerated cellulose membrane with 30 kDa NMWCO delivers retentate recovery of greater than 90%.

2 Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 5 minutes).

3 Heat-sealed membrane minimizes downstream extractables.

4 100% integrity tested for reliable performance.

5 Convenient sample monitoring with translucent housing and volume gradations.

6 Direct pipette sample access eliminates processing step to recover concentrate.

7 High concentration factors of 80–100X.

Physical form：

Color Code: Clear with pink cover