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Material: polyethylene cap,polyethylene liner,polypropylene tube,regenerated cellulose membrane,regenerated cellulose support,styrene-butadiene housing
Quality Level: 400
Sterility: non-sterile
Product line: Amicon®
Manufacturer/tradename:Amicon® Ultra
Parameter: 4 mL sample volume
Technique(s):protein extraction: suitable, protein purification: suitable
L: 12.1cm (4.807in.)
Diam.: 2.97 cm
Filtration area: 7.6cm2
Volume: 15mL
Pore size:3kDa NMWCO
Shipped in: ambient
General description:
Amicon® Ultra 15mL centrifugal devices with Ultracel® 3,000 Dalton molecular weight cutoff filters provide fast ultrafiltration, and reliable retention of macromolecules. For example, Cytochrome C (12.4 kDa) at 0.25 mg/ml initial concentration is retained at >90% .
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.
The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. With their vertical membrane design and deadstop, Amicon® Ultra 15mL filters deliver the highest recovery with short spin times.
Application:
1 Ultrafiltration with 3,000 Dalton molecular weight cutoff Ultracel® membrane.
2 Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
3 Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLC.
4 Concentration of dilute or pre-purified proteins from column eluents.
5 Desalting, buffer exchange and protein dialysis.
Features and Benefits:
1 Ultracel® regenerated cellulose membrane with 3kDa NMWCO delivers retentate recovery of greater than 90%.
2 Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 10–15 minutes).
3 Heat-sealed membrane minimizes downstream extractables.
4 100% integrity tested for reliable performance.
5 Convenient sample monitoring with translucent housing and volume gradations.
6 Direct pipette sample access eliminates processing step to recover concentrate.
7 High concentration factors of 80–100X.
Color Code: Clear with pink cap
