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My Cart> Antibodies > Loading Control Antibodies > Cell/Cytoplasmic Protein > Anti-GAPDH Monoclonal AntibodyReactivity：Human
Application：WB,IF
Clone Type：Monoclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：2597
Host：Mouse
Qty：
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| Name | Anti-GAPDH Monoclonal Antibody |
| Host | Mouse |
| Clone Type | Monoclonal Antibody |
| Subtype | IgG1 |
| Dilution Ratio | WB 1:2000-5000.IF 1:100-200. |
| Target | GAPDH |
| Background | Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. As well as functioning as a glycolytic enzyme in cytoplasm, recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of data appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is expressed in all cells. It is constitutively expressed in almost all tissues at high levels. There are however some physiological factors such as hypoxia and diabetes that increase GAPDH expression in certain cell types. GAPDH molecule is composed of four 36kDa subunits. |
| Swiss Prot | P04406 |
| Gene ID | 2597 |
| Subcellular Location | Cytoskeleton Cytosol Nucleus |
| Immunogen | Recombinant Protein |
| Calculated MW | 32/36kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Human |
| Application | WB IF |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with GAPDH antibody diluted at 1:2000;Lane: HeLa,293T,A549,HepG2.
Immunofluorescence analysis of SH-SY5Y cells using GAPDH antibody diluted at 1:100.
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