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My CartReactivity：Human,Rat,Mouse
Application： WB,IHC,ELISA
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Host：Rabbit
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| Name | Anti-NTAQ1 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:1000-3000. IHC 1:50-200. |
| Target | NTAQ1 |
| Background | Mediates the side-chain deamidation of N-terminal glutamine residues to glutamate, an important step in N-end rule pathway of protein degradation. Conversion of the resulting N-terminal glutamine to glutamate renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. Does not act on substrates with internal or C-terminal glutamine and does not act on non-glutamine residues in any position. Does not deaminate acetylated N-terminal glutamine. With the exception of proline, all tested second-position residues on substrate peptides do not greatly influence the activity. In contrast, a proline at position 2, virtually abolishes deamidation of N-terminal glutamine. |
| Swiss Prot | Q96HA8 |
| Gene ID | 55093 |
| Subcellular Location | Cytoplasm Nucleus |
| Immunogen | Recombinant protein of human NTAQ1 |
| Public Immunogen Range | 1-205aa |
| Calculated MW | 17/24kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Piece | Piece |
| Y-Dilution Ratio | WB 1:1000-3000. IHC 1:50-200. ELISA 1:8000-80000. |
| Specification | 50ul 100ul |
| Reactivity | Human Rat Mouse |
| Application | WB IHC ELISA |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Immunohistochemistry of paraffin-embedded Human thyroid cancer using NTAQ1 antibody diluted at 1:100
Immunohistochemistry of paraffin-embedded Human colorectal cancer using NTAQ1 antibody diluted at 1:100
Immunohistochemistry of paraffin-embedded Human prostate cancer using NTAQ1 antibody diluted at 1:100
Western blot analysis with NTAQ1 antibody diluted at 1:2000;Lane:A431
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