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My Cart> Antibodies > Loading Control Antibodies > Cell/Cytoplasmic Protein > Anti-GAPDH Polyclonal AntibodyReactivity：Mouse,Rat,Monkey,Human
Application：IF,IP,WB,IHC
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：2597
Host：Rabbit
Qty：
Size：
| Name | Anti-GAPDH Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:8000-12000. IHC 1:50-200. IF 1:50-200. IP 1:200. |
| Target | GAPDH |
| Background | Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. It catalyzes an important energy-yielding step in carbohydrate metabolism, thereversible oxidative phosphorylation of glyceraldehyde-3-phosphatein the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). Recent evidence suggests that it also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export (1). GAPDH has also been implicated in playing a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer’s and Huntington’s disease (2). GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots. |
| Swiss Prot | P04406 |
| Gene ID | 2597 |
| Subcellular Location | Cytoplasm Nucleus |
| Immunogen | Recombinant protein of human GAPDH |
| Public Immunogen Range | 1-336aa |
| Calculated MW | 32/36kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Human Monkey Rat Mouse |
| Application | IP WB IHC IF |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with GAPDH antibody diluted at 1:8000;Lane: HEK293,HeLa,293T,HepG2,COS7,A431,Mouse liver,Mouse kidney,Rat liver.
Immunohistochemistry of paraffin-embedded Human breast cancer with GAPDH antibody diluted at 1:100.
Immunofluorescence analysis of HeLa cells using GAPDH antibody diluted at 1:100.
Immunoprecipitation analysis of 1mg Hela extracts using GAPDH antibody(IP:1:200;WB:1:10000).Control IgG: Normal Rabbit IgG.
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