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CAS:25535-16-4
Storage:Powder:2-8℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year
Purity:≥98%
Appearance:Red Solid
Examples of using this product（for reference only）
In Vitro:
Cell （KB cells；5μg/mL PI；15min）:
PI and FDA co-staining experiments evaluated the effect of PTT In Vitro KB cells were cultured with Au NCs in 6-well plate at concentration of 100μg/mL based on the content of Au, and then irradiated by 660 nm laser with laser power density of 2 W/cm2 for different times of 0, 5, 10 and 15 min. FDA of 5μg/mL was used to stain the living cells, and the staining time was 15 s. Meanwhile, PI of 5 μg/mL was used to stain the apoptosis cells, and the staining time was 15 min.
Reference：
Chen Q, Guan G, Deng F, Yang D, Wu P, Kang S, Sun R, Wang X, Zhou D, Dai W, Wang X, Zhang H, He B, Chen D, Zhang Q. Anisotropic active ligandations in siRNA-Loaded hybrid nanodiscs lead to distinct carcinostatic outcomes by regulating nano-bio interactions. Biomaterials. 2020 Apr 3;251:120008. doi: 10.1016/j.biomaterials.2020.120008. Epub ahead of print. PMID: 32388031.
P. ternata seedling(Pinellia ternata root tip cell; 5 μg/mL PI; 10min):
We accurately weigh 100 mg of fluorescein diacetate (FDA) (Solarbio, Beijing, China) and dissolved it in 4 mL of acetone to prepare a 25 mg·mL-1 mother liquor, which was stored away from light at -20 ℃. When in use, FDA storage solution was added to 0.65 mol·L-1 mannitol to obtain FDA dyeing solution in proportion. 10 mg of propidium iodide (PI) (Solarbio, China) was dissolved in 2 mL of 0.65 mol·L-1 mannitol to prepare a 5 mol·L-1 mother liquor. The FDA (15 μg·mL-1) and PI (5 μg·mL-1) mixtures were used to treat P. ternata seedlings. P. ternata seedlings were hydroponically cultured and treated with M-Pa-B for 1, 2, 3, and 4 h. After that, the root tips of seedlings were washed three times with deionized water and then dyed with the FDA-PI in the dark for 10 min. The excess dye was washed away thoroughly with deionized water after dyeing. Ten root samples were collected from five individual plants at each time point. A laser confocal scanning microscope was used for observation and photography. The excitation and emission wavelengths are 488 nm and 630 nm, respectively.
Reference：
He Z, Wang Y, Yan Y, Qin S, He H, Mao R, Liang Z. Dynamic analysis of physiological indices and transcriptome profiling revealing the mechanisms of the allelopathic effects of phenolic acids on Pinellia ternata. Front Plant Sci. 2022 Oct 18;13:1039507. doi: 10.3389/fpls.2022.1039507. PMID: 36340387; PMCID: PMC9635339.
