Gene Name |
RPA2 |
English Name |
replication protein A2, 32kDa |
Chinese Name |
复制蛋白A2 |
Uniprot |
P15927 |
GeneID |
6118 |
Predicted Cross Reactivity |
Human Mouse Rat (Fruit fly Gorilla A.thaliana) |
氨基酸大小 |
29kDa |
表达氨基酸 |
1-270aa |
Background |
This gene encodes a subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and co-ordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase.The RPA complex protects single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors.The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which oligonucleotide/oligosaccharide-binding (OB) domains of the complex are utilized, and differing in the length of DNA bound.This subunit contains a single OB domain that participates in high-affinity DNA binding and also contains a winged helix domain at its carboxy terminus, which interacts with many genome maintenance protein.Post-translational modifications of the RPA complex also plays a role in co-ordinating different damage response pathways. |
Application |
Immunology research |
Source |
E.coli |
Purification |
NI-NTA affinity purification |
Formulation |
Lyophilized from a 0.2 μm filtered solution of Urea, Tris-HCl, PH8.0. |
Delivery Condition |
The product is shipped at -20°C temperature. Upon receipt, store it immediately at the temperature listed below. |
Storage |
Reconstituted protein solution can be stored at 4-7°C for 1-2 months, stable at < -20°C for 2-3 years. |
Reconstitution |
Reconstituted protein solution can be diluted with distilled PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Purity |
Purity≥85% as determined by reducing SDS-PAGE. |
Endotoxin |
/ |
Bioactive |
Not tested. |
Tag information |
With a 6His tag at the N-terminus. |
Unit |
Piece |
Specification |
10ug 50ug 100ug 1mg |