Gene Name |
RAB3A |
English Name |
RAB3A, member RAS oncogene family |
Chinese Name |
RAB3A,RAS致癌基因家族成员 |
Uniprot |
P20336 |
GeneID |
5864 |
Predicted Cross Reactivity |
Human Mouse Rat (Pig Bovine) |
氨基酸大小 |
25kDa |
表达氨基酸 |
1-220aa |
Background |
Small GTP-binding protein that plays a central role in regulated exocytosis and secretion.Controls the recruitment, tethering and docking of secretory vesicles to the plasma membrane.Upon stimulation, switches to its active GTP-bound form, cycles to vesicles and recruits effectors such as RIMS1, RIMS2, Rabphilin-3A/RPH3A, RPH3AL or SYTL4 to help the docking of vesicules onto the plasma membrane.Upon GTP hydrolysis by GTPase-activating protein, dissociates from the vesicle membrane allowing the exocytosis to proceed.Stimulates insulin secretion through interaction with RIMS2 or RPH3AL effectors in pancreatic beta cells.Regulates calcium-dependent lysosome exocytosis and plasma membrane repair (PMR) via the interaction with 2 effectors, SYTL4 and myosin-9/MYH9.Acts as a positive regulator of acrosome content secretion in sperm cells by interacting with RIMS1.Plays also a role in the regulation of dopamine release by interacting with synaptotagmin I/SYT. |
Application |
Immunology research |
Source |
E.coli |
Purification |
NI-NTA affinity purification |
Formulation |
Lyophilized from a 0.2 μm filtered solution of Urea, Tris-HCl, PH8.0. |
Delivery Condition |
The product is shipped at -20°C temperature. Upon receipt, store it immediately at the temperature listed below. |
Storage |
Reconstituted protein solution can be stored at 4-7°C for 1-2 months, stable at < -20°C for 2-3 years. |
Reconstitution |
Reconstituted protein solution can be diluted with distilled PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Purity |
Purity≥85% as determined by reducing SDS-PAGE. |
Endotoxin |
/ |
Bioactive |
Not tested. |
Tag information |
With a 6His tag at the N-terminus. |
Unit |
Piece |
Specification |
10ug 50ug 100ug 1mg |