Gene Name |
SFRS1 |
English Name |
splicing factor, arginine/serine-rich 1 |
Chinese Name |
剪接因子,精氨酸/丝氨酸丰富1 |
Uniprot |
Q07955 |
GeneID |
6426 |
Predicted Cross Reactivity |
Human Mouse (Bovine Pig Gorilla Chicken) |
氨基酸大小 |
28kDa |
表达氨基酸 |
1-248aa |
Background |
Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing.Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF.Can stimulate binding of U1 snRNP to a 5'-splice site-containing pre-mRNA.Binds to purine-rich RNA sequences, either the octamer, 5'-RGAAGAAC-3' (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC.Binds preferentially to the 5'-CGAGGCG-3' motif in vitro.Three copies of the octamer constitute a powerful splicing enhancer in vitro, the ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing.Isoform ASF-2 and isoform ASF-3 act as splicing repressors.May function as export adapter involved in mRNA nuclear export through the TAP/NXF1 pathway. |
Application |
Immunology research |
Source |
E.coli |
Purification |
NI-NTA affinity purification |
Formulation |
Lyophilized from a 0.2 μm filtered solution of Urea, Tris-HCl, PH8.0. |
Delivery Condition |
The product is shipped at -20°C temperature. Upon receipt, store it immediately at the temperature listed below. |
Storage |
Reconstituted protein solution can be stored at 4-7°C for 1-2 months, stable at < -20°C for 2-3 years. |
Reconstitution |
Reconstituted protein solution can be diluted with distilled PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Purity |
Purity≥85% as determined by reducing SDS-PAGE. |
Endotoxin |
/ |
Bioactive |
Not tested. |
Tag information |
With a 6His tag at the N-terminus. |
Unit |
Piece |
Specification |
10ug 50ug 100ug 1mg |