Gene Name |
DTL |
English Name |
denticleless homolog (Drosophila) |
Chinese Name |
无齿蛋白质同系物 |
Uniprot |
Q9NZJ0 |
GeneID |
51514 |
Predicted Cross Reactivity |
Human Mouse (Chicken) |
氨基酸大小 |
39kDa |
表达氨基酸 |
1-350aa |
Background |
Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis.The DCX(DTL) complex, also named CRL4(CDT2) complex, mediates the polyubiquitination and subsequent degradation of CDT1, CDKN1A/p21(CIP1), FBH1, KMT5A and SDE2.CDT1 degradation in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication.CDKN1A/p21(CIP1) degradation during S phase or following UV irradiation is essential to control replication licensing.KMT5A degradation is also important for a proper regulation of mechanisms such as TGF-beta signaling, cell cycle progression, DNA repair and cell migration.Most substrates require their interaction with PCNA for their polyubiquitination: substrates interact with PCNA via their PIP-box, and those containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to their degradation.In undamaged proliferating cells, the DCX(DTL) complex also promotes the 'Lys-164' monoubiquitination of PCNA, thereby being involved in PCNA-dependent translesion DNA synthesis.The DDB1-CUL4A-DTL E3 ligase complex regulates the circadian clock function by mediating the ubiquitination and degradation of CRY1. |
Application |
Immunology research |
Source |
E.coli |
Purification |
NI-NTA affinity purification |
Formulation |
Lyophilized from a 0.2 μm filtered solution of Urea, Tris-HCl, PH8.0. |
Delivery Condition |
The product is shipped at -20°C temperature. Upon receipt, store it immediately at the temperature listed below. |
Storage |
Reconstituted protein solution can be stored at 4-7°C for 1-2 months, stable at < -20°C for 2-3 years. |
Reconstitution |
Reconstituted protein solution can be diluted with distilled PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Purity |
Purity≥85% as determined by reducing SDS-PAGE. |
Endotoxin |
/ |
Bioactive |
Not tested. |
Tag information |
With a 6His tag at the N-terminus. |
Unit |
Piece |
Specification |
10ug 50ug 100ug 1mg |