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My CartReactivity：Mouse,Human,Monkey,Rat
Application： IHC,WB
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Host：Rabbit
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| Name | Anti-MCM3 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:1000-3000. IHC 1:100-300. |
| Target | MCM3 |
| Background | Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation. |
| Swiss Prot | P25205 |
| Gene ID | 4172 |
| Subcellular Location | Nucleus |
| Immunogen | Recombinant protein of human MCM3 |
| Public Immunogen Range | 459-809aa |
| Calculated MW | 91/96kDa |
| Purification Method | Octanoic acid-ammonium sulfate precipitation |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Piece | Piece |
| Specification | 50ul 100ul |
| Reactivity | Human Monkey Rat Mouse |
| Application | WB IHC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with MCM3 antibody diluted at 1:2000;Lane:HeLa,293T,PC-12,NIH/3T3,COS-7,HepG2
Immunohistochemistry of paraffin-embedded Human cervical cancer with MCM3 antibody diluted at 1:200
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