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My CartCAS：1047644-62-1
Storage：Powder:-20℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year
Purity：≥98%
Appearance：White to off-white Solid
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| CAS | 1047644-62-1 |
| Name | Afuresertib |
| Molecular Formula | C18H17Cl2FN4OS |
| Molecular Weight | 427.32 |
| Solubility | Soluble in DMSO ≥5mg/mL(Need ultrasonic) |
| Purity | ≥98% |
| Appearance | White to off-white Solid |
| Storage | Powder:-20℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year |
| EC | EINECS 1592732-453-0 |
| MDL | MFCD26961098 |
| SMILES | O=C(C1=CC(C2=C(Cl)C=NN2C)=C(Cl)S1)N[C@@H](CC3=CC=CC(F)=C3)CN |
| Target Point | Akt |
| Passage | PI3K/Akt/mTOR |
| Background | Afuresertib is a potent Akt inhibitor. |
| Biological Activity | Afuresertib 是一种有效的 ATP 竞争性的特异性 Akt 抑制剂。[1] |
| In Vitro | Afuresertib对恶性胸膜间皮瘤(MPM)细胞具有良好的肿瘤抑制作用。 Afuresertib显著增加ACC-MESO-4和MSTO-211H细胞中caspase-3和caspase-7活性以及凋亡细胞数量。 Afuresertib强烈阻止细胞周期进入G1期。 Western blotting分析表明，Afuresertib可增加p21WAF1 / CIP1的表达，降低Akt底物的磷酸化，包括GSK-3β和FOXO家族蛋白。 Afuresertib诱导的p21表达通过诱导FOXO活性促进G1期阻滞。 Afuresertib显著增强顺铂诱导的细胞毒性。 Afuresertib调节E2F1和MYC的表达，这与成纤维细胞核心血清反应有关[1]。 |
| Cell Experiment | 将MPM细胞接种在96孔板中(细胞密度，2.5×103细胞/孔)，并在37℃下孵育24小时。接下来，将细胞在含有指定浓度的Akt抑制剂(例如，Afuresertib; 50，20，10，5，2，1，0.5，0.2，0.1和0.01μM)的培养基中温育72小时。接下来，将MTT溶液添加到每个孔中，并将细胞孵育4小时。最后，将细胞与裂解缓冲液(10％SDS在0.01mol / L氯化氢中)一起温育过夜。使用SpectraMAX M5分光光度计[1]在550nm处测量吸光度。 |
| Data Literature Source | [1]. Yamaji M，et al. Novel ATP-competitive Akt inhibitor Afuresertib suppresses the proliferation of malignant pleural mesothelioma cells. Cancer Med. 2017 Nov;6(11):2646-2659 |
| Unit | Bottle |
| Specification | 2mg 5mg |
是一种有效的Akt 抑制剂。
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
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