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Application：ELISA
Reactivity：Human
Sample Type：Serum，Plasma，Cell Culture Supernatant
Sensitivity：300 pg/ml
Storage：2-8℃，6 months
Gene ID：3956
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| Name | Human galectin-1 ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | Galectin-1 |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Human |
| Application | ELISA |
| Reaction Type | Sandwich-ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 625-40000 pg/ml |
| Sensitivity | 300 pg/ml |
| Specificity | Do not react with Human Amphiregulin， Human Betacellulin， Human EGF， Human EGFR， Human HB-EGF， Human HRG-α， Human SMDF274 etc. |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Assay Time | 3h |
| Swiss Prot | P09382 |
| Gene ID | 3956 |
| Detection Principle | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗人 galectin-1单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的人 galectin-1会与酶标板上的包被抗体充分结合；洗板后加入HRP酶标记的抗人 galectin-1抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的人 galectin-1发生特异性结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的人 galectin-1，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的人 galectin-1浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中人 galectin-1的浓度。 |
| Background | The galectins constitute a large family of carbohydrate-binding proteins that function in many systems both intracellularly and following secretion. Galectins contain either one or two carbohydrate recognition domains (CRR) which mediate recognition of N-acetyl-lactosamine-containing glycoproteins. Some galectins exist in multiple isoforms due to alternative splicing. Individual galectins differ in their tissue distribution and in their carbohydrate-binding specificities. |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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请问这个试剂盒可以检测小鼠galectin1吗？ 2023-02-03 13:11:21
Administrator您好，这个试剂盒是用来检测人源样本的，ELISA试剂盒大多数是区分种属，检测小鼠galectin1的试剂盒货号为SEKM-0082。