Determination of Significance：

Nucleotides have important biological functions. They are a class of compounds composed of three substances: purine base or pyrimidine base, ribose or deoxyribose, and phosphoric acid. They are mainly involved in the formation of nucleosides.  Adenosine triphosphate (ATP) is considered to be a universal energy source that is essential for cell synthesis in the survival and reproduction of all organisms. ATP can be produced through a variety of cellular pathways. The most typical example is synthesis by adenosine triphosphate synthase through oxidative phosphorylation in mitochondria, or synthesis by photosynthesis in plant chloroplasts. The main energy sources for ATP synthesis are glucose and fatty acids.Adenosine diphosphate (ADP) is widely present in animals, plants, microorganisms and cultured cells. In organisms, ADP is a product of breaking a high-energy phosphate bond (ATP) ydrolyzed to lose a phosphate radical) and releasing energy. Adenosine monophosphate (AMP) is widely found in animals, plants, microorganisms and cultured cells. It is formed after ATP and ADP release energy in the body. It can continue to bind phosphate groups to form adenosine diphosphate (ADP) and adenosine triphosphate (ATP). It is the product of incomplete hydrolysis of ATP.

Measurement Principle：

ATP、ADP、AMP have an absorption peak at 254 nm, and its content can be determined by high performance liquid chromatography.

Self Provided：

High-efficiency liquid chromatograph (C18 column (4.6×250 mm), ultraviolet detector (VWD)), desktop centrifuge, adjustable pipette, mortar/ homogenizer, brown EP tube, 50 syringe filters (water, 0.45 μm), syringe, suction filter, filter membrane (organic, water), 50 brown injection bottle (2 mL), acetonitrile (chromatographically pure, 500 mL), ultrapure water.

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