Superoxide Dismutase（SOD）Activity Assay Kit （WST-1 Method）
Cat.No：BC5160 Solarbio
Storage：Store at 2-8℃，6 months
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> Biochemical Assay Kits > REDOX Series > Anti-Reverse Series > Superoxide Dismutase（SOD）Activity Assay Kit （WST-1 Method）
Product Information
| Name | Superoxide Dismutase（SOD）Activity Assay Kit （WST-1 Method） |
| Detection Method | Spectrophotometer |
| Storage | Store at 2-8℃，6 months |
| Unit | Kit |
| Specification | 50T/24S |
Determination of Significance：
Superoxide dismutase (SOD, EC 1.15.1.1) is a kind of metalloenzyme widely found in organism. It is an important oxygen radical scavenger and can catalytic disproportionation of superoxide anion to form H2O2 and O2. SOD is not only the superoxide anion scavenging enzyme, but also the main H2O2 producing enzyme, which plays an important role in the biological antioxidant system.
Measurement Principle：
Superoxide anion (O2-) is produced by xanthine and xanthine oxidase reaction system. O2- can reduce tetrazolium-1(WST-1) to form a water-soluble yellow formazan dye, which has absorbance in 450 nm. SOD can remove O2- and inhibit the formation of the formazan dye. The darker the yellow color of the reaction solution, the lower the SOD activity. The lighter the yellow color of the reaction solution, the higher the activity of SOD.
Self Provided：
Spectrophotometer, table centrifuge,water bath/constant temperature foster box, transferpettor, 1 mL glass cuvette, mortar/homogenizer, ice and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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