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Storage:Store at 2-8℃，6 months
Determination of Significance：
Superoxide dismutase (SOD, EC 1.15.1.1) is a kind of metalloenzyme widely found in organism. It is an important oxygen radical scavenger and can catalytic disproportionation of superoxide anion to form H2O2 and O2. SOD is not only the superoxide anion scavenging enzyme, but also the main H2O2 producing enzyme, which plays an important role in the biological antioxidant system.
Measurement Principle：
Superoxide anion (O2-) is produced by xanthine and xanthine oxidase reaction system. O2- can reduce tetrazolium-1(WST-1) to form a water-soluble yellow formazan dye, which has absorbance in 450 nm. SOD can remove O2- and inhibit the formation of the formazan dye. The darker the yellow color of the reaction solution, the lower the SOD activity. The lighter the yellow color of the reaction solution, the higher the activity of SOD.
Self Provided：
Spectrophotometer/microplate reader, table centrifuge, water bath/constant temperature foster box, electronic balance, transferpettor, micro glass cuvette/96 well flat-bottom plate, mortar/ homogenizer, ice and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
这个的超氧化物歧化酶是总超氧化物歧化酶嘛2022-09-23 13:33:28
Administrator检测的是总超氧化物歧化酶的活性
我想做革兰氏阳性菌SOD酶的提取分析，但因为有较厚的细胞壁，通常超声破碎不完全，我可以通过加入溶菌酶的方法进行辅助破胞吗，是否会对最终的检测结果造成影响2022-07-25 15:31:42
Administrator您好，生化试剂盒在样本处理过程中不建议用酶消化处理或引入其他试剂，推荐用物理的方法去破碎样本，革兰氏阳性菌这种可以提高超声功率，不超过300W，以及增加超声次数对样本破碎，注意如果提高超声功率或者延长超声时间间隔时间要延长以及做好预冷，减少超声过程中产热对酶活的影响