Determination of Significance：

Wine is a general term for alcoholic (ethanol) beverages, and ethanol is the main component of wine and one of the important indicators to measure the quality of wine. Ethanol can be used in the manufacture of acetic acid, beverages, flavors, dyes, fuels, etc. Ethanol with a volume fraction of 70% to 75% is commonly used as a disinfectant in medicine. Ethanol has a wide range of uses in the chemical industry, medical and health, food industry, agricultural production and other fields.

Measurement Principle：

Ethanol is oxidized under the catalysis of alcohol oxidase to produce hydrogen peroxide. Peroxidase catalyzes the oxidation of hydrogen peroxide to 4-aminoantipyrine to couple phenol to generate a colored compound with a characteristic absorption peak at 505nm. The change of the absorption peak at 505nm can be measured to reflect the ethanol content.

Self Provided：

Spectrophotometer, desk centrifuge, constant temperature incubator/water bath, pipette, 1mL glass cuvette, mortar/homogenizer, ice and distilled water.

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