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My CartReactivity：Rat,Monkey,Human,Mouse
Application： WB,IHC
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Host：Rabbit
Qty：
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| Name | Anti-ADD1 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Predicted Reactivity | Chimpanzee |
| Dilution Ratio | WB 1:200-1000. IHC 1:50-200. |
| Target | ADD1 |
| Background | Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described. |
| Swiss Prot | P35611 |
| Gene ID | 118 |
| Subcellular Location | Cytoplasm |
| Immunogen | A synthetic peptide of human ADD1 |
| Public Immunogen Range | 555-605/737aa |
| Calculated MW | 56/70/73/80/84kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Piece | Piece |
| Specification | 50ul 100ul |
| Reactivity | Human Monkey Rat Mouse |
| Application | WB IHC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with ADD1 antibody diluted at 1:500;Lane: Vero,A431,HeLa,,K-562.
Immunohistochemistry of paraffin-embedded Human thyroid cancer with ADD1 antibody diluted at 1:100.
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