Gene Name |
SPAG5 |
English Name |
sperm associated antigen 5 |
Chinese Name |
精子相关抗原5 |
Uniprot |
Q96R06 |
GeneID |
10615 |
Predicted Cross Reactivity |
Human Mouse |
氨基酸大小 |
38.3kDa |
表达氨基酸 |
10-360aa |
Background |
Essential component of the mitotic spindle required for normal chromosome segregation and progression into anaphase (PubMed:11724960, PubMed:12356910, PubMed:27462074).Required for chromosome alignment, normal timing of sister chromatid segregation, and maintenance of spindle pole architecture (PubMed:17664331, PubMed:27462074).In complex with SKAP, promotes stable microtubule-kinetochore attachments. May contribute to the regulation of separase activity. May regulate AURKA localization to mitotic spindle, but not to centrosomes and CCNB1 localization to both mitotic spindle and centrosomes (PubMed:18361916, PubMed:21402792).Involved in centriole duplication. Required for CDK5RAP2, CEP152, WDR62 and CEP63 centrosomal localization and promotes the centrosomal localization of CDK2 (PubMed:26297806).In non-mitotic cells, upon stress induction, inhibits mammalian target of rapamycin complex 1 (mTORC1) association and recruits the mTORC1 component RPTOR to stress granules (SGs), thereby preventing mTORC1 hyperactivation-induced apoptosis (PubMed:23953116).May enhance GSK3B-mediated phosphorylation of other substrates, such as MAPT/TAU (PubMed:18055457). |
Application |
Immunology research |
Source |
E.coli |
Purification |
NI-NTA affinity purification |
Formulation |
Lyophilized from a 0.2 μm filtered solution of Urea, Tris-HCl, PH8.0. |
Delivery Condition |
The product is shipped at -20°C temperature. Upon receipt, store it immediately at the temperature listed below. |
Storage |
Reconstituted protein solution can be stored at 4-7°C for 2-3 months, stable at < -20°C for 1-2 years. |
Reconstitution |
Reconstituted protein solution can be diluted with distilled PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Purity |
Purity≥85% as determined by reducing SDS-PAGE. |
Bioactive |
Not tested. |
Tag information |
With a 6His tag at the N-terminus. |
Unit |
Piece |
Specification |
10ug 50ug 100ug 1mg |