| CAS |
59-49-4 |
| Chinese Name |
2-苯并唑啉酮 |
| English Name |
2-Benzoxazolinone |
| Synonyms |
2-Hydroxybenzoxazole;1，3-Benzoxazol-2(3H)-one |
| Molecular Formula |
C7H5NO2 |
| Molecular Weight |
135.12 |
| Solubility |
Soluble in DMSO |
| Purity |
≥98% |
| Appearance |
Light brown to brown Solid |
| Storage |
Powder:-20℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year |
| EC |
EINECS 200-430-0 |
| MDL |
MFCD00005716 |
| SMILES |
C1=CC=C2C(=C1)NC(=O)O2 |
| InChIKey |
ASSKVPFEZFQQNQ-UHFFFAOYSA-N |
| InChI |
InChI=1S/C7H5NO2/c9-7-8-5-3-1-2-4-6(5)10-7/h1-4H,(H,8,9) |
| PubChem CID |
6043 |
| Target Point |
Parasite |
| Passage |
Anti-infection |
| Background |
It is an anti-leishmania reagent. |
| Biological Activity |
2-Benzoxazolinone (BOA), a well-known allelochemical with strong phytotoxicity, is a potential herbicidal candidate.[1] 2-Benzoxazolinone is an anti-leishmanial agent with an LC50 of 40 μg/mL against L. donovani[2] |
| IC50 |
LC50: 40μg/mL(L. donovani)[2] |
| In Vitro |
BOA induces oxidative stress in mung bean as indicated by increased generation of H2O2，enhanced LP and accumulation of endogenous PRO. It is accompanied by an upregulation of the activities of various scavenging enzymes—SODs，peroxidases，CATs and GR—in roots and leaves of mung bean in response to BOA exposure.[1] The LC50 values for BOA and pentamidine are 40 pg/ml and 39.8 pg/mi，respectively. BOA showed antileishmanial activity and compares well with pentamidine. [2] |
| Cell Experiment |
Promastigote para- sites in the stationary phase of growth(1 x io parasite/mi)were inoculated into culture tubes containing BHI-agar as solid part and HBSS as hquid part. To these tubes different concentrations(10 to 100 pg/mi，DMSO/PBS)of BOA and pentamidine were added，respectively. The inhibitory effect of BOA was compared with pentamidine which is known to be active against leishmaniasis，and with untreated control cultures. The whole operation was carried out aseptically in an ultraviolet chamber and the tubes were incubated for 5 days at 22 °C. The toxicity of DMSO was determined by adding different amounts of solvent(0.1 to 1.5%)to suspensions of promastigotes under the conditions described above. After five days the value of the non-toxic dilution was found to be 0.6% and growth inhibition(up to 15%)was observed between 0.6% and 1 %; therefore，higher concentrations of DMSO were never used. Antileishmanial activity of each of the com_x0002_pounds was determined by counting the number of live parasites per field microscopically. The LC55 values were calculated by probit analysis and no mortallty was observed in mice when BOA was administered((p.)in the doses ranging from 0.25—1 g/kg. The mice were kept under observation for one week.[2] |
| Kinase Experiment |
For the BOA treatment，we followed the method used by Burgos and Talbert，wherein seeds germinated on a filter paper disc moistened with BOA and grow until the measurement of seedling length. Seeds of mung bean were surface sterilized with sodium hypochlorite(0.1%，w/v)followed by washing with distilled water three times. These were subjected to germination in 15 cm diameter Petri dishes lined with a Whatman no. 1 filter circle and moistened with 7 ml of respective BOA treatment solution or distilled water(control). The 7 ml of the 0.1，0.5，1.0，2.5，and 5.0 mM BOA solution used cor_x0002_respond to nearly 0.0946，0.473，0.946，2.365，4.73 mg ml–1 BOA，respectively. It was in the range similar to that used by Burgos and Talbert [13]. Twenty-five mung bean seeds were used in each Petri dish. Five replicates were maintained for each treatment in a completely randomized manner. All the Petri dishes were placed in a seed germinator maintained at 25 ± 2 °C，75 ± 3% relative humidity and a 16 h:8 h light: dark photoperiod with a photosynthetic photon flux density of 150 μmol m–2 s–1. After 7 days，root and shoot length of emerged seedlings from each treatment were measured. Further，roots and leaves from each treatment were harvested and stored at –80 °C prior to enzyme extraction and other estimations.[1] |
| Data Literature Source |
[1] Batish D R ， Singh H P ， Setia N ，et al. 2-Benzoxazolinone (BOA) induced oxidative stress，lipid peroxidation and changes in some antioxidant enzyme activities in mung bean (Phaseolus aureus).[J]. Plant Physiol Biochem，2006，44(11-12):819-827.
[2]. Kapil A，et al. Leishmanicidal activity of 2-benzoxazolinone from Acanthus illicifolius in vitro. Planta Med. 1994 Apr;60(2):187-8. |
| Unit |
Bottle |
| Specification |
50mg 100mg 250mg |
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