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CAS:33864-99-2
Appearance:Purple to black solid
Storage:Powder : 2-8℃, 2 years; In solvent(mother liquid): -20℃, 1 month; -80℃, 6 months
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Alcian blue (Alcian), also known as Aithian blue or Alcin blue, is a kind of copper titanium cyanine conjugated dye. This cationic dye combines with acidic groups, namely Alcin blue, to form insoluble complexes with anionic groups contained in tissues such as carboxyl and sulfate. Alysin blue is formed by a central coppery phthalocyanine ring connected to four isothiourea groups by a thioether bond. The isothiourea group is moderately alkaline, making Alcian blue ribbon cationic. The exact mechanism by which Alcian blue dyes carbohydrates is unknown, but it is generally believed that the cationic isothiourea is electrostatically linked to polyanions in tissues. For example, the carboxyl group and sulfate group of the acidic mucous substance containing carboxyl group and sulfate group form an insoluble complex, that is, the positively charged salt bond in the dye molecule and the negatively charged acid group in the acidic mucous substance are combined in blue. The different pH values of the dye solution can be used to distinguish the group of mucous substances: at a pH of 2.5, the carboxyl group in the tissue ionizes, carries a negative charge, forms a salt bond with the cations in the Alysin blue, and stains tissues with carboxyl groups (such as proteoglycan/hyaluronic acid and epithelial acidic mucin). At a pH value of 1.0, the sulfate in the tissue ionizes, carrying a negative charge, and forms a salt bond with the cations in Alcian blue, staining the tissue with sulfate (such as the sulfate mucous substance). Neutral mucins (such as those found in the gastric mucosa and Brunner glands) do not react with Alcian blue. In cartilage staining, when pH was 1, COOH did not stain, and OSO3H stained. When pH was 2.5, COOH staining was good, while sulfate mucin staining was poor, thus staining sulfate mucin and salivary mucin. This reagent is only suitable for scientific research, not for clinical diagnosis or other purposes. Usage (for reference only)
1 Modified Lison method: bone marrow tablets were fixed with methanol for 5 min, washed and air-dried; Alcian blue solution (10 g/L Alcian blue aqueous solution 100 mL and 1% acetic acid 100 mL mixed and filtered) was dyed for 30 min and fully washed; 1 g/L neutral red water solution was re-dyed for 30 s, washed, air dried, and examined under microscope. 2 Mowry method: Fixed bone marrow tablets for 5 min and washed with water; Alcian blue solution (Alcian blue 1g, 3% acetic acid 100 mL) was dyed for 5 ~ 10 min, fully washed, 1g /L neutral red water oozing solution was re-dyed for 30 s, washed, air dried, and examined under microscope. 3 Lev method: Fresh smear fixed with methanol for 5 min and washed with water; Alycian blue solution (Alycian blue 1 g, 0.1 mol/L hydrochloric acid 100 mL) was dyed for 10 ~ 30 min, and fully washed. 1 g/L neutral red water solution was re-dyed for 30 s, washed, air dried, and examined under microscope.
