Determination of Significance:
Direct bilirubin (DBIL) is also called conjugated bilirubin. After indirect bilirubin enters the liver, it is combined with glucuronic acid by the action of glucuronosyltransferase in the liver. The increase of direct bilirubin is of great significance for clinical diagnosis of obstructive jaundice, hepatocellular jaundice, liver cancer, pancreatic head cancer, cholelithiasis and cholangiocarcinoma.
Measurement Principle:
Direct bilirubin could be oxidized by sodium nitrite to form biliverdin, which has absorbance in 450 nm. The content of direct bilirubin can be calculated by detecting the wavelength change at 450 nm.
Self Provided:
Spectrophotometer/Microplate reader, desk centrifuge, constant temperature foster box/water-bath, pipette, micro glass cuvette/96 well flat-bottom plate, ice and distilled water.
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