Determination of Significance:
Total bilirubin (TBIL) is the sum of direct bilirubin and indirect bilirubin. Determination of serum total bilirubin is an important item in liver and gallbladder function tests. The content of direct bilirubin is of great significance for clinical diagnosis of latent jaundice.
Measurement Principle:
Total bilirubin could be oxidized by sodium nitrite to form biliverdin, which has absorbance in 450 nm. The content of total bilirubin can be calculated by detecting the wavelength change at 450 nm.
Self Provided:
Spectrophotometer/Microplate reader, desk centrifuge, constant temperature foster box/water-bath, pipette, micro glass cuvette/96 well flat-bottom plate, ice and distilled water.
Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.