Anti-EIF2S3 Polyclonal Antibody
Cat.No:K111214P Solarbio
Reactivity:Rat,Human,Mouse
Application: WB,IHC
Storage:Store at -20℃. Avoid freeze/thaw cycles.
Host:Rabbit
Qty:
Size:
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My CartReactivity:Rat,Human,Mouse
Application: WB,IHC
Storage:Store at -20℃. Avoid freeze/thaw cycles.
Host:Rabbit
Qty:
Size:
English Name | Anti-EIF2S3 Polyclonal Antibody |
Host | Rabbit |
Clone Type | Polyclonal Antibody |
Subtype | IgG |
Predicted Reactivity | Bovine;Zebrafish;Orangutan;Chicken;Rabbit;Pig |
Dilution Ratio | WB 1:1000-3000. IHC 1:50-200. |
Target | EIF2S3 |
Background | As a subunit of eukaryotic initiation factor 2 (eIF-2), involved in the early steps of protein synthesis. In the presence of GTP, eIF-2 forms a ternary complex with initiator tRNA Met-tRNAi and then recruits the 40S ribosomal complex and initiation factors eIF-1, eIF-1A and eIF-3 to form the 43S pre-initiation complex (43S PIC), a step that determines the rate of protein translation. The 43S PIC binds to mRNA and scans downstream to the initiation codon, where it forms a 48S initiation complex by codon-anticodon base pairing. This leads to the displacement of eIF-1 to allow GTPase-activating protein (GAP) eIF-5-mediated hydrolysis of eIF2-bound GTP. Hydrolysis of GTP and release of Pi, which makes GTP hydrolysis irreversible, causes the release of the eIF-2-GDP binary complex from the 40S subunit, an event that is essential for the subsequent joining of the 60S ribosomal subunit to form an elongation-competent 80S ribosome. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must be exchanged with GTP by way of a reaction catalyzed by GDP-GTP exchange factor (GEF) eIF-2B. Along with its paralog on chromosome Y, may contribute to spermatogenesis up to the round spermatid stage. |
Swiss Prot | P41091 |
Gene ID | 1968 |
Subcellular Location | Cytoplasm |
Immunogen | Recombinant protein of human EIF2S3 |
Public Immunogen Range | 123-472aa |
Calculated MW | 51kDa |
Purification Method | Affinity purification |
Storage Buffer | PBS with 50% Glycerol,0.03% Proclin300,0.5% BSA,pH 7.3. |
Storage | Store at -20℃. Avoid freeze/thaw cycles. |
Piece | Piece |
Specification | 50ul 100ul |
Reactivity | Human Rat Mouse |
Application | WB IHC |
Remark:These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder:Quick spin before use. If you have any questions, please contact us.
Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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