{{cart_num}}
My CartReactivity：Pig,Rat,Mouse,Human
Application： ELISA,FCM,IF,IHC,WB
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Host：Mouse
Qty：
Size：
| English Name | Anti-NF-H/NF200 Monoclonal Antibody |
| Chinese Name | 高分子量神经丝蛋白抗体 |
| Host | Mouse |
| Clone Type | Monoclonal Antibody |
| Subtype | IgG2a |
| Dilution Ratio | WB 1:5000-50000. IHC 1:4000-16000. IF 1:20-200. |
| Target | NF-H/NF200 |
| Background | NEFH, also named as KIAA0845 and NFH, Belongs to the intermediate filament family. It has an important function in mature axons that is not subserved by the two smaller NF proteins. Neurofilaments are the 10nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M,145-160 kDa and NF-H, 200-220 kDa. This antibody recognize NEFH only. |
| Swiss Prot | P12036 |
| Gene ID | 4744 |
| Subcellular Location | Cell projection Cytoplasm Cytoskeleton Intermediate filament |
| Immunogen | Recombinant protein of human NF200 |
| Public Immunogen Range | 1-214aa |
| Calculated MW | 112Da |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Piece | Piece |
| Specification | 50ul 100ul |
| Reactivity | Pig Rat Mouse Human |
| Application | ELISA FCM IF IHC WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with NF-H antibody diluted at 1:10000;Lane: Pig cerebellum.
Immunohistochemistry of paraffin-embedded rat cerebellum using NF-H antibody diluted at 1:8000.
Immunofluorescence analysis of rat brain using NF-H antibody diluted at 1:400.
Sorry, there is no more information.
Manual Download
