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Your Position: Home > Small Molecule Compounds > Dye & Probe > Fura-2 AM
Fura-2 AM

CAS:108964-32-5

Appearance:Solid

Storage:Powder : -20℃, 2 years;In solvent(mother liquid): -20℃, 1 month; -80℃, 6 months

Purity:≥95%

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Fura-2 AM
Cat.No：
IF1510
Brand：
Solarbio
SKU Tongzhou Beijing Haidian Beijing Wuhan Guangzhou
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Product Information Related Products Related Paper COA Technical Q&A

FURA-2AM is a high affinity calcium ion fluorescent probe that is permeable to cells. FURA-2AM is an ester form of FURA-2, a highly polar acidic compound that cannot enter living cells, and the addition of acetoxymethyl (AM) to its negative group significantly increases cell permeability. After penetrating the cell membrane into living cells, FURA-2AM is de-esterified by intracellular esterase into FURA-2 free acid, which binds specifically to cytoplasmic free Ca2+ (binding ratio 1:1). When FURA-2 binds to free Ca2+ in the cytoplasm, it exhibits an absorption shift, with the excitation wavelength peak changing from 380 nm to 340 nm, while the emission peak near 510 nm remains unchanged. There was a quantitative relationship between fluorescence intensity and the concentration of bound Ca2+. Fura-2 is generally excited at 340 nm (calcium ion -Fura 2) and 380nm (Fura 2) wavelengths, and the intracellular calcium ion concentration is calculated by using the ratio of fluorescence intensity corresponding to the two excitations. Ratio measurements minimize the effects of photobleaching in the mixture, leakage of fluorescent probes, uneven loading of probes and differences in cell thickness, providing more reliable and reproducible results.

Remark：
These protocols are for reference only. Solarbio does not independently validate these methods.

Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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