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CAS:11089-65-9
Storage:Powder:-20℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year
Purity:≥98%
Appearance:White to light yellow Solid
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Tunicamycin is an antibiotic that inhibits n-glycosylation and blocks GlcNAc phosphotransferase (GPT). Tunicamycin causes the accumulation of unfolded proteins in the endoplasmic reticulum (ER) of cells and induces ER stress, and leads to blocked DNA synthesis and G1 phase cell cycle arrest. Itamycin is A mixture of homologous nucleoside antibiotics, a mixture of four isomers A, B, C, and D: Isomer A, n=8, C37H60N4O16, 816.90 Isomer B, n=9, C38H62N4O16, 830.93 Isomer C, n=10, C39H64N4O16, 844.95 isomer D, n=11, C40H66N4O16, 858.99
Examples of using this product（for reference only）
In Vitro:
Cell（Buffalo Ovarian Granulosa Cells; 1~5 μg/mL tunicamycin; 37℃, 5% CO2, 4–48 h）：
Briefly, the culture medium was removed and replaced with the culture medium consisting of diluted tunicamycin (TM) (Solarbio, Beijing, China). GCs were then subjected to increasing doses of TM at concentrations of 1, 1.5, 2, 2.5, and 5 μg/mL and cultured at 37℃ for approximately 24–48 h in a 5% CO2 incubator.
Reference：
Yang W, Liu R, Sun Q, Huang X, Zhang J, Huang L, Zhang P, Zhang M, Fu Q. Quercetin Alleviates Endoplasmic Reticulum Stress-Induced Apoptosis in Buffalo Ovarian Granulosa Cells. Animals (Basel). 2022 Mar 20;12(6):787. doi: 10.3390/ani12060787. PMID: 35327186; PMCID: PMC8944572.
Cell（ human NSCLC cell lines A549, PC9, and H1299；0.5-2 μM Tunicamycin/TM；24-72h）：
0.5, 1, and 2 μM TM were used for 48 h or 2 μM TM was used for 24, 48, and 72 h to induce ER stress. Ten micromolar MG132 was used for 6 h prior to harvesting the cells, and 50 μg·mL?1 CHX was used for indicated time to detect protein degradation. Ten micromolar Radezolid was used to inhibit RRBP1 expression.
Reference：
Wang W, Wang M, Xiao Y, Wang Y, Ma L, Guo L, Wu X, Lin X, Zhang P. USP35 mitigates endoplasmic reticulum stress-induced apoptosis by stabilizing RRBP1 in non-small cell lung cancer. Mol Oncol. 2022 Apr;16(7):1572-1590. doi: 10.1002/1878-0261.13112. Epub 2021 Oct 18. PMID: 34618999; PMCID: PMC8978513.
