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Determination of Significance：
Nitrate nitrogen is the nitrogen element contained in nitrate. Nitrate nitrogen in soil is one of the main forms of nitrogen absorption by higher plants, and its content is directly related to the yield and quality of crops.
Measurement Principle：
Under the condition of concentrated acid, NO3- reacts with salicylic acid to form nitrosalicylic acid, which is yellow under the condition of alkaline (pH>12), and its color is directly proportional to the content, so the content of nitrate nitrogen can be calculated.
Self Provided：
Spectrophotometer/ microplate reader, table centrifuge, water-bath, micro glass cuvette/96 well flat-bottom plate, transferpettor, oscillator and distilled water, concentrated H2SO4.
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